Clock gene expression levels and relationship with clinical and pathological features in colorectal cancer patients.
Identifieur interne : 000A96 ( Main/Exploration ); précédent : 000A95; suivant : 000A97Clock gene expression levels and relationship with clinical and pathological features in colorectal cancer patients.
Auteurs : G. Mazzoccoli [Italie] ; A. Panza ; M R Valvano ; O. Palumbo ; M. Carella ; V. Pazienza ; G. Biscaglia ; F. Tavano ; P. Di Sebastiano ; A. Andriulli ; A. PiepoliSource :
- Chronobiology international ; 2011.
English descriptors
- KwdEn :
- Aged, CLOCK Proteins (genetics), CLOCK Proteins (metabolism), Colorectal Neoplasms (metabolism), Colorectal Neoplasms (pathology), Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic (physiology), Humans, Male, Microsatellite Instability, Middle Aged, RNA, Messenger (genetics), RNA, Messenger (metabolism).
- MESH :
- chemical , genetics : CLOCK Proteins, RNA, Messenger.
- chemical , metabolism : CLOCK Proteins, RNA, Messenger.
- metabolism : Colorectal Neoplasms.
- pathology : Colorectal Neoplasms.
- physiology : Gene Expression Regulation, Neoplastic.
- Aged, Female, Gene Expression Profiling, Humans, Male, Microsatellite Instability, Middle Aged.
Abstract
The clock gene machinery controls cellular metabolism, proliferation, and key functions, such as DNA damage recognition and repair. Dysfunction of the circadian clock is involved in tumorigenesis, and altered expression of some clock genes has been found in cancer patients. The aim of this study was to evaluate the expression levels of core clock genes in colorectal cancer (CRC). Quantitative real-time polymerase chain reaction (qPCR) was used to examine ARNTL1, CLOCK, PER1, PER2, PER3, CRY1, CRY2, Timeless (TIM), TIPIN, and CSNK1? expression levels in the tumor tissue and matched apparently healthy mucosa of CRC patients. In the tumor tissue of CRC patients, compared to their matched healthy mucosa, expression levels of ARNTL1 (p=.002), PER1 (p=.002), PER2 (p=.011), PER3 (p=.003), and CRY2 (p=.012) were lower, whereas the expression level of TIM (p=.044) was higher. No significant difference was observed in the expression levels of CLOCK (p=.778), CRY1 (p=.600), CSNK1 (p=.903), and TIPIN (p=.136). As to the clinical and pathological features, a significant association was found between low CRY1 expression levels in tumor mucosa and age (p=.026), and female sex (p=.005), whereas high CRY1 expression levels in tumor mucosa were associated with cancer location in the distal colon (p?=?.015). Moreover, high TIM mRNA levels in the tumor mucosa were prevalent whenever proximal lymph nodes were involved (p= .013) and associated with TNM stages III-IV (p=.005) and microsatellite instability (p=.015). Significantly poorer survival rates were evidenced for CRC patients with lower expression in the tumor tissue of PER1 (p=.010), PER3 (p= .010), and CSNKIE (p=.024). In conclusion, abnormal expression levels of core clock genes in CRC tissue may be related to the process of tumorigenesis and exert an influence on host/tumor interactions.
DOI: 10.3109/07420528.2011.615182
PubMed: 22080729
Affiliations:
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Le document en format XML
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Mazzoccoli</name><affiliation wicri:level="1"><nlm:affiliation>Department of Internal Medicine and Chronobiology Unit, Scientific Institute and Regional General Hospital CasaSollievo della Sofferenza, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG), Italy. g.mazzoccoli@tin.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Internal Medicine and Chronobiology Unit, Scientific Institute and Regional General Hospital CasaSollievo della Sofferenza, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG)</wicri:regionArea><wicri:noRegion>San Giovanni Rotondo (FG)</wicri:noRegion></affiliation></author><author><name sortKey="Panza, A" sort="Panza, A" uniqKey="Panza A" first="A" last="Panza">A. Panza</name></author><author><name sortKey="Valvano, M R" sort="Valvano, M R" uniqKey="Valvano M" first="M R" last="Valvano">M R Valvano</name></author><author><name sortKey="Palumbo, O" sort="Palumbo, O" uniqKey="Palumbo O" first="O" last="Palumbo">O. Palumbo</name></author><author><name sortKey="Carella, M" sort="Carella, M" uniqKey="Carella M" first="M" last="Carella">M. Carella</name></author><author><name sortKey="Pazienza, V" sort="Pazienza, V" uniqKey="Pazienza V" first="V" last="Pazienza">V. Pazienza</name></author><author><name sortKey="Biscaglia, G" sort="Biscaglia, G" uniqKey="Biscaglia G" first="G" last="Biscaglia">G. Biscaglia</name></author><author><name sortKey="Tavano, F" sort="Tavano, F" uniqKey="Tavano F" first="F" last="Tavano">F. Tavano</name></author><author><name sortKey="Di Sebastiano, P" sort="Di Sebastiano, P" uniqKey="Di Sebastiano P" first="P" last="Di Sebastiano">P. 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Mazzoccoli</name><affiliation wicri:level="1"><nlm:affiliation>Department of Internal Medicine and Chronobiology Unit, Scientific Institute and Regional General Hospital CasaSollievo della Sofferenza, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG), Italy. g.mazzoccoli@tin.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Internal Medicine and Chronobiology Unit, Scientific Institute and Regional General Hospital CasaSollievo della Sofferenza, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG)</wicri:regionArea><wicri:noRegion>San Giovanni Rotondo (FG)</wicri:noRegion></affiliation></author><author><name sortKey="Panza, A" sort="Panza, A" uniqKey="Panza A" first="A" last="Panza">A. Panza</name></author><author><name sortKey="Valvano, M R" sort="Valvano, M R" uniqKey="Valvano M" first="M R" last="Valvano">M R Valvano</name></author><author><name sortKey="Palumbo, O" sort="Palumbo, O" uniqKey="Palumbo O" first="O" last="Palumbo">O. Palumbo</name></author><author><name sortKey="Carella, M" sort="Carella, M" uniqKey="Carella M" first="M" last="Carella">M. Carella</name></author><author><name sortKey="Pazienza, V" sort="Pazienza, V" uniqKey="Pazienza V" first="V" last="Pazienza">V. Pazienza</name></author><author><name sortKey="Biscaglia, G" sort="Biscaglia, G" uniqKey="Biscaglia G" first="G" last="Biscaglia">G. Biscaglia</name></author><author><name sortKey="Tavano, F" sort="Tavano, F" uniqKey="Tavano F" first="F" last="Tavano">F. Tavano</name></author><author><name sortKey="Di Sebastiano, P" sort="Di Sebastiano, P" uniqKey="Di Sebastiano P" first="P" last="Di Sebastiano">P. Di Sebastiano</name></author><author><name sortKey="Andriulli, A" sort="Andriulli, A" uniqKey="Andriulli A" first="A" last="Andriulli">A. Andriulli</name></author><author><name sortKey="Piepoli, A" sort="Piepoli, A" uniqKey="Piepoli A" first="A" last="Piepoli">A. Piepoli</name></author></analytic><series><title level="j">Chronobiology international</title><idno type="e-ISSN">1525-6073</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>CLOCK Proteins (genetics)</term><term>CLOCK Proteins (metabolism)</term><term>Colorectal Neoplasms (metabolism)</term><term>Colorectal Neoplasms (pathology)</term><term>Female</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Neoplastic (physiology)</term><term>Humans</term><term>Male</term><term>Microsatellite Instability</term><term>Middle Aged</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>CLOCK Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>CLOCK Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Colorectal Neoplasms</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Colorectal Neoplasms</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Neoplastic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Female</term><term>Gene Expression Profiling</term><term>Humans</term><term>Male</term><term>Microsatellite Instability</term><term>Middle Aged</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The clock gene machinery controls cellular metabolism, proliferation, and key functions, such as DNA damage recognition and repair. Dysfunction of the circadian clock is involved in tumorigenesis, and altered expression of some clock genes has been found in cancer patients. The aim of this study was to evaluate the expression levels of core clock genes in colorectal cancer (CRC). Quantitative real-time polymerase chain reaction (qPCR) was used to examine ARNTL1, CLOCK, PER1, PER2, PER3, CRY1, CRY2, Timeless (TIM), TIPIN, and CSNK1? expression levels in the tumor tissue and matched apparently healthy mucosa of CRC patients. In the tumor tissue of CRC patients, compared to their matched healthy mucosa, expression levels of ARNTL1 (p=.002), PER1 (p=.002), PER2 (p=.011), PER3 (p=.003), and CRY2 (p=.012) were lower, whereas the expression level of TIM (p=.044) was higher. No significant difference was observed in the expression levels of CLOCK (p=.778), CRY1 (p=.600), CSNK1 (p=.903), and TIPIN (p=.136). As to the clinical and pathological features, a significant association was found between low CRY1 expression levels in tumor mucosa and age (p=.026), and female sex (p=.005), whereas high CRY1 expression levels in tumor mucosa were associated with cancer location in the distal colon (p?=?.015). Moreover, high TIM mRNA levels in the tumor mucosa were prevalent whenever proximal lymph nodes were involved (p= .013) and associated with TNM stages III-IV (p=.005) and microsatellite instability (p=.015). Significantly poorer survival rates were evidenced for CRC patients with lower expression in the tumor tissue of PER1 (p=.010), PER3 (p= .010), and CSNKIE (p=.024). In conclusion, abnormal expression levels of core clock genes in CRC tissue may be related to the process of tumorigenesis and exert an influence on host/tumor interactions.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Andriulli, A" sort="Andriulli, A" uniqKey="Andriulli A" first="A" last="Andriulli">A. Andriulli</name><name sortKey="Biscaglia, G" sort="Biscaglia, G" uniqKey="Biscaglia G" first="G" last="Biscaglia">G. Biscaglia</name><name sortKey="Carella, M" sort="Carella, M" uniqKey="Carella M" first="M" last="Carella">M. Carella</name><name sortKey="Di Sebastiano, P" sort="Di Sebastiano, P" uniqKey="Di Sebastiano P" first="P" last="Di Sebastiano">P. Di Sebastiano</name><name sortKey="Palumbo, O" sort="Palumbo, O" uniqKey="Palumbo O" first="O" last="Palumbo">O. Palumbo</name><name sortKey="Panza, A" sort="Panza, A" uniqKey="Panza A" first="A" last="Panza">A. Panza</name><name sortKey="Pazienza, V" sort="Pazienza, V" uniqKey="Pazienza V" first="V" last="Pazienza">V. Pazienza</name><name sortKey="Piepoli, A" sort="Piepoli, A" uniqKey="Piepoli A" first="A" last="Piepoli">A. Piepoli</name><name sortKey="Tavano, F" sort="Tavano, F" uniqKey="Tavano F" first="F" last="Tavano">F. Tavano</name><name sortKey="Valvano, M R" sort="Valvano, M R" uniqKey="Valvano M" first="M R" last="Valvano">M R Valvano</name></noCountry><country name="Italie"><noRegion><name sortKey="Mazzoccoli, G" sort="Mazzoccoli, G" uniqKey="Mazzoccoli G" first="G" last="Mazzoccoli">G. Mazzoccoli</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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